Why It Is Still Considerate As The Gold Standard For Dna...

This technique is still very useful overall because of its low error range, and also is the reason why it is still considerate as the Gold standard for DNA sequencing, among other things to validate NGS data (Pareek Smoczynski 2011). Aiming to reduce costs and improve quality and efficiency, the New Generation Sequencers appeared. With the surging of NGS technologies, today it is viable to sequence in a particular subject with a suspected genetic disease or with tendency to that compound disease, up to thousands of genes at the same time (Pareek Smoczynski 2011). There are different existing methods, however all shared similar features about Massive Parallel sequencing reactions. All of them required a pre-determined library sample preparation through DNA break-up and amplification by custom adaptors. Each library fragment is amplified on a solid surface (cell flow) by covalent-attached DNA linkers (oligonucleotides) which hybridize the library adaptors. This amplification creates millions of DNA clusters, each originated from a single library fragment. Each fragment will act as a single sequencing reaction. After this, each machine provides a raw data at the end of the sequencing run. This run data is a collect ion of DNA sequences that were generated of each cluster. At the moment, the most broadly used targeted sequencing method is maybe the Whole Exome Sequencing (WES) since is the known genome protein-coding region and represents no more than 2% of the geneticShow MoreRelatedDeveloping Management Skills404131 Words   |  1617 PagesEditorial Director: Sally Yagan Editor in Chief: Eric Svendsen Acquisitions Editor: Kim Norbuta Editorial Project Manager: Claudia Fernandes Director of Marketing: Patrice Lumumba Jones Marketing Manager: Nikki Ayana Jones Senior Marketing Assistant: Ian Gold Senior Managing Editor: Judy Leale Senior Production Project Manager: Kelly Warsak Senior Operations Supervisor: Arnold Vila Operations Specialist: Ilene Kahn Senior Art Director: Janet Slowik Interior Design: Suzanne Duda and Michael Fruhbeis PermissionsRead MoreManagement Course: Mba−10 General Management215330 Words   |  862 PagesManaging Change 121 121 147 147 Text 3. Why Organizations Change Text Cohen †¢ Effective Behavior in Organizations, Seventh Edition 14. Initiating Change 174 174 Text iii Cases 221 221 225 The Consolidated Life Case: Caught Between Corporate Cultures Who’s in Charge? (The)(Jim)(Davis)(Case) Morin−Jarrell †¢ Driving Shareholder Value I. Valuation 229 229 253 279 1. The Value−Based Management Framework: An Overview 2. Why Value Value? 4. The Value Manager Harvard